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Biorbyt新产品:新型重组SARS-CoV-2 Spike蛋白,D614G突变体

发布时间:2021-03-04 10:43 点击次数:

Biorbyt新产品:新型重组SARS-CoV-2 Spike蛋白,D614G突变体

原创 biorbyt 英国biorbyt 1周前
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#新冠产品 10 

 

新冠发展现状

The original Wuhan strain of COVID-19 is no longer the dominant circulating virus in the global pandemic. A variant carrying a D614G mutation in the Spike (S) protein of SARS-CoV-2 emerged at an early stage of the pandemic and is now one of the prevalent forms(1).

武汉原始COVID-19株已不再是全球大流行中的主要循环病毒。在大流行的早期出现的一种在SARS-CoV-2的Spike(S)蛋白中带有D614G突变的变体,现在已成为流行的新冠病毒形式之一(1)。

 

Korber et al reported that this D614G variant may be more virulent with increased patient viral loads but is not associated with a more severe disease outcome. According to Grubaugh et al, the impact of this mutation on the global pandemic is yet unknown and will require further investigation (2).

Korber等研究者报道,这种新冠D614G突变体可能会随着患者病毒载量的增加而更具毒性,但与更严重的疾病结果无关。根据Grubaugh等研究者的报道,这种突变对全球大流行的影响尚不清楚,需要进一步研究(2)。

 

图片来自Korber et al, 2020

 

SARS-CoV-2 Spike 蛋白 D614G 突变

Biorbyt are proud to announce the launch of a novel recombinant full-length trimeric soluble SARS-CoV-2 Spike protein D614G variant: A soluble recombinant protein with a foldon trimerization motif, mutated Furin recognition site and 2 stabilising mutations (K986P and V987P), based on/modified from Amanat et al, 2020 (3).

Biorbyt现推出新型重组全长三聚体可溶性SARS-CoV-2 Spike蛋白D614G变体:一种具有foldon三聚化基序,突变的弗林蛋白酶识别位点和2个稳定突变(K986P和V987P)的可溶性重组蛋白,基于Amanat等人(2020)的修改 (3)。

 

产品名称:Human SARS-CoV-2 trimeric soluble full-length Spike protein产品货号:orb668935产品链接:https://www.biorbyt.com/human-sars-cov-2-trimeric-solu-orb668935.html产品信息如下:

 

 

 

了解D614G 突变

The D614G mutation is in the S1 subunit of the Spike protein. The S protein monomer consists of two active subunits, the S1 subunit which binds the host receptor and the S2 subunit which facilitates fusion of viral and host cell membranes. The S protein monomers combine to form the distinctive homotrimers which project from the viral cell surface. Viral entry into the host cell is via the ACE2 receptors located on the host cell membrane (4,5). The spike protein is highly immunogenic and most of the vaccines and therapeutic agents currently in development target this region.

D614G突变位于Spike蛋白的S1亚基中。S蛋白单体由两个活性亚基组成,S1亚基结合宿主受体,而S2亚基促进病毒和宿主细胞膜的融合。S蛋白单体结合形成从病毒细胞表面突出的独特同三聚体。病毒通过位于宿主细胞膜上的ACE2受体进入宿主细胞(4,5)。刺突蛋白具有高度的免疫原性,目前正在开发的大多数疫苗和治疗剂均靶向该区域。

 

产品质控

The highly validated recombinant full-length trimeric SARS-CoV-2 Spike protein, D614G variant (amino acids 16-1213) is produced in HEK293 cells and supplied in liquid format. Endotoxin levels are less than 0.1 ng/μg (1 IEU/μg), and purity >90% as estimated by SDS-PAGE. A C-terminal 8x histidine Tag has been added to the protein for ease of use.

经过高度验证的重组全长三聚体SARS-CoV-2 Spike蛋白D614G变体(氨基酸16-1213)在HEK293细胞中生产并以可溶解形式提供。内毒素水平小于0.1 ng /μg(1 IEU /μg),通过SDS-PAGE估算纯度> 90%。为了易于使用,已将C端8x组氨酸标签添加到蛋白质中。

 

有该产品采购需求的老师请联系biorbyt中国办事处咨询:400-966-3101;027-87663101。

  

 

参考资料:

1. Korber et al., Tracking Changes in SARS-CoV-2 Spike: Evidence that D614G Increases Infectivity of the COVID-19 Virus, Cell (2020), https://doi.org/10.1016/j.cell.2020.06.043

2. Grubaugh et al., Making Sense of Mutation: What D614G Means for the COVID-19 Pandemic Remains Unclear, Cell (2020), https://doi.org/10.1016/j.cell.2020.06.040

3. Amanat, F., Stadlbauer, D., Strohmeier, S., et al. A serological assay to detect SARS-CoV-2 seroconversion in humans. Nat Med., 2020;26:1033–1036.

4. Walls et al., Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein 2020, Cell, 180, 281–292.

5. Hoffmann M., Kleine-Weber H., Schroeder S., et al. SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell, 2020;181(2):271-280


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